Summary/Abstract: 5 points
· One short paragraph (3-4 sentences at most) summarizing the experimental goals (hypotheses) and your group results.
Background: 5 pts
· Summarize the background information presented to you in the first pre-lab lecture about Chlamydomonas. You do not need to include the information about microscopy.
· Identify the question that you are asking:
o Identify the drug that you tested and how that drug works (ie what process does it affect)
o Then identify the research question we are asking in this activity
Materials and Methods (5 pts)
· Summarize the experimental procedure that you followed for your group.
o If you did something that was not in the standard procedure, describe it here as well.
o This summary needs to be in paragraph form
o This summary needs to be in YOUR words (do not just copy the methods from the protocol—this is a summary).
Results (10 pts)
· Present your data in a table or chart—this should include the time points and the flagellar length (or not) for both the control and treatment.
· Summarize, in paragraph form, the results that you saw.
Week 1 :
Gabriela Urbina
Tre Sheffield
Kunjan P
Lab notebook pt2
Week 1: Basic lab Protocol for flagella in Chlamydomonas Reinhardtill.
Methods: add in protocol: ……
Results
Under the 4 x, there was visibility of color, but to zoom in, we used the 40 x to see clearly through the scope.
2. While on 40 x and moving to Ph1, there is more outline on the organisms, and they look a little darker. We can see the structure more clearly.
3……………………………………ph2 ( there is no photo ) for this one….
4 The Ph3 looks less visible and the color is dim. The organisms are moving more frequently.
Discovered :
After our first part of the procedure, we established that the flagella was not visible for any of the accommodations to the microscope.
5
After adding Iodine ….( Lugols
Results …
40x on A: Supper tiny specs.
PH1 ON 40X: Still no flagella, and based on our view, there is a dark line around the organisms. They are still clustered, but the movement has died down, and they just seem to be floating.
4. PH2 ( 40x ) The flagella is more visible, and the light on full power blinds the visibility of the organism. There is no longer movement.
5. PH3 (40X )No flagella visible and the organisms are no longer moving.
10 total:
Length of the Flagella
1 – 0.05 mm ( 50 uL)
2- 0.05 mm ( 50 uL)
3- 0.07mm (70 uL)
4- 0.06 mm ( 60uL)
5- 0.04 mm (40 uL)
6- 0.03 mm ( 30 uL)
7- 0.08 mm (80 mL)
8- 0.06 mm (60 uL)
9- 0.08 mm (80uL )
10 – 0.07 mm (70 uL )
Conclusion:
3 /6/2024 Week 2: regeneration of flagella in chlamydonas reinhardtii
Procedure : The drug we are using is: Actinomycin D
Add the procedure……..
Review from Last Week:
Measurements: measurements of the flagella
Organism 1 Orgamism 2 Organism 3
|
1- 1: 0.05 mm |
1)-2: 0.02 mm |
1)-0.07 mm |
|
2-2: 0.03 mm |
2) -0.02 mm |
2)- 0.04 mm |
Time “ 0” Samples:
|
Positive (+) |
1-1: 0.07 mm |
1:2: 0.07mm |
2-1: 0.1mm |
2:2: 0.11mm |
3:1 – 0.3 mm |
3-2: 0.2 mm |
4:1- 0.05 mm |
4:2- 0.07mm |
5:1- 0.06mm |
5:2- 0.04mm |
|
Negative (-) |
1:1- 0.04mm |
1:2- 0.05 mm |
2:1- 0.06mm |
2:2 0.06 mm |
3:1- 0.0 |
3:2- 0.00mm |
4:1- 0.08 mm |
4:2- 0.05mm |
5:1 – 0.04 mm |
5:2- 0.06 mm |
Time: “12”
|
Positive (+) |
1:1- 0.04 mm |
1:2- 0.06mm |
2:1- 0.1 mm |
2:2- 0.04 mm |
3:1- 0.06 mm |
3:2- 0.05mm |
4:1- 0.05mm |
4:2- 0 (one fell off) |
5:1 – 0.07 mm |
5:2- 0.06mm |
|
Negative (-) |
1:1- 0.02mm |
1:2- 0.03mm |
2:1- 0.03mm |
2:2- 0.03mm |
3:1- 00 |
3:2- 00 |
4:1 0.05mm |
4:2 0.04mm |
5:1- 0.07mm |
5:2- 0.05mm |
There is pieces of flagella breaking off and floating.
Time “24”
|
Organism |
1:1 |
1:2 |
2:1 |
2:2 |
3:1 |
3:2 |
4:1 |
4:2 |
5:1 |
5:2 |
|
Positive (+) |
0.1mm |
0.11m |
00 |
00 |
0.06mm |
0.05mm |
0.02mm |
0.03mm |
0.1mm |
0.04mm |
|
Negative (-) |
0.05mm |
0.05mm |
0 |
0 |
00 |
00 |
0.02mm |
0.03mm |
0.0mm |
0.05mm |
There is a continuation of alot less flagella;there was detached flagella surrounding.
Time “36” ( broken flaggella ) (it was delayed )
|
Organism |
1:1 |
1:2 |
2:1 |
2:2 |
3:1 |
3:2 |
4:1 |
4:2 |
5:1 |
5:2 |
|
Positive (+) |
0.08- mm |
0.08mm |
00mm |
00mm |
0.06mm |
0.05mm |
0.05mm |
0.05mm |
0.01mm |
0.01mm |
|
Negative (-) |
00 |
00 |
00 |
00 |
0.05mm |
0.06mm |
0.03mm |
0.04mm |
0.00 |
0.00 |
Dr.c said this during lab 3/20/2024 ( this is so tre can see this ) later on we can delete this…..
It was not an imidient It could have been a mixing issue. It gives them some other things, but the fact that I had one group see or start to see the Regeneration, so it's going to be hard, quite honestly, for you guys to see and write about the effect of the drug because the whole process of the acid shock happened later so I am going to take that into account and this is why I wanted to have this conversation before we started today's activity the lab report I still want you
Not everybody's results but your groups results okay we're still going to use that same metric. Still, you'll see in the directions that while I'm going to have you write up your results, I'm either going to have you analyze other data or data similar to what you might have expected to evaluate. I will have different sets of data whether you were signed cycle has mine. This is why I asked this question and whether you assign. Now that I know this
Time “48”
|
Organism |
1:1 |
1:2 |
2:1 |
2:2 |
3:1 |
3:2 |
4:1 |
4:2 |
5:1 |
5:2 |
|
Postive (+) |
0.02mm |
0.02mm |
0.02mm |
0.02 |
0.04 |
0.04 |
0.01 |
0.01 |
00 |
00 |
|
Negative (-) |
00 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0.03mm |
0.04mm |
Time “60”
|
Organism |
1:1 |
1;2 |
2;1 |
2:2 |
3:1 |
3:2 |
4;1 |
4:2 |
5:1 |
5:2 |
|
Positive (+) |
0.7 |
0.4mm |
0.6 |
0.6mm |
00 |
00 |
0.7 |
0.4 |
0.8 |
0.6 |
|
Negative (-) |
0 |
0 |
0 |
0 |
0 |
0 |
0 |